Jpn. J. Infect. Dis., 56, 151-155, 2003

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Original Article

Multiplex Polymerase Chain Reaction Assay for Selective Detection of Salmonella enterica Serovar Typhimurium

Young-Hee Lim, Kenji Hirose1*, Hidemasa Izumiya1, Eiji Arakawa1, Hideyuki Takahashi1, Jun Terajima1, Ken-ichiro Itoh2, Kazumichi Tamura1, Sung-Il Kim3 and Haruo Watanabe1

Department of Clinical Laboratory Science, College of Health Sciences, Korea University and 3Department of Clinical Pathology, Korea University Anam Hospital, Korea University, Seoul 136-703, Korea, 1Department of Bacteriology, National Institute of Infectious Diseases, Tokyo 162-8640 and 2Bacteriological Laboratory Training Division, Infectious Disease Surveillance Center, National Institute of Infectious Diseases, Tokyo 208-0011, Japan

(Received May 6, 2003. September 4, 2003)

*Corresponding author: Mailing address: Department of Bacteriology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan. Tel: +81-3-5285-1111, Fax: +81-3-5285-1163, E-mail: hirosek@nih.go.jp


SUMMARY: A multiplex polymerase chain reaction (PCR) assay was developed for the identification of Salmonella enterica serovar Typhimurium. Three sets of primers were designed for detecting O4, H:i, and H:1,2 antigen genes from the antigen-specific genes rfbJ, fliC, and fljB, respectively. These were evaluated in a multiplex PCR assay by using DNAs from S. enterica serovar Typhimurium, 15 other Salmonella serovars, and 8 non-Salmonella enteric pathogens. Multiplex PCR proved to be capable of identifying S. enterica serovar Typhimurium specifically and differentiating it from other Salmonella serovars in addition to non-Salmonella enteric pathogens. Thus, this multiplex PCR assay can be practically applied to the identificaiton of S. enterica serovar Typhimurium.

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