Jpn. J. Infect. Dis., 56, 161-164, 2003
To see a printabler version of the article in the Adobe file format, click this [PDF] link.
Short Communication
Evaluation of a Rapid Serological Chromatographic Immunoassay for the Diagnosis of Pulmonary Tuberculosis in Accra, Ghana
Andrew Anthony Adjei*, Henry Armah, Owusu Achaw Duah1, Theophilus Adiku2, and Ian Fiifi Adukwei Hesse3
Department of Pathology, 1Department of Chemical Pathology, 2Department of Microbiology and 3Department of Medicine, University of Ghana Medical School, Accra, Ghana.
(Received February 24, 2003. Accepted August 11, 2003)
*Corresponding author: Mailing address: Department of Pathology, University of Ghana Medical School, P. O. Box 4236, Accra, Ghana. Tel: +233-21-670266, Fax: +233-21-668286, E-mail: andrewadjei50@hotmail.com
SUMMARY: Advances in serologic assays for tuberculosis
(TB) have made serology an attractive surveillance tool. The sensitivity,
specificity, and predictive value of these new assays have been
well established. We employed an immunochromatographic serodiagnostic
test for detecting antibodies (immunoglobulin [Ig] G, IgA,
and IgM) specific for mycobacteria-specific antigen 60 (A60) in
the serum of suspected TB patients (n=310) attending the
Chest Clinic of the Korle-Bu Teaching Hospital, Accra, Ghana.
Compared to the gold standard, sputum culture, the performance
indexes of the test kit indicated a sensitivity of 80% and a specificity
of 98.2%, respectively. The positive predictive value and the
likelihood ratios for positive and negative results were 96.6,
44.4, and 0.2%, respectively, when compared to sputum culture.
Our results suggest that the serodiagnostic test kit may be a
valuable tool for the diagnosis of TB, and can provide simple
and satisfactory results, particularly in cases in which TB is
clinically difficult to diagnose by the usual clinical and laboratory
tests.
Go to JJID Homepage Go to JJID 56 (4) Contents