Jpn. J. Infect. Dis., 57, 25-28, 2004

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Short Communication

Isolation of a Promoter Region of a Secreted Metalloprotease Gene from Microsporum canis

Tsuyoshi Yamada^1,2, Koichi Makimura^1,2*, Asuka Hirai³, Rui Kano³, Atsuhiko Hasegawa³, Katsuhisa Uchida^1,2 and Hideyo Yamaguchi¹,2

¹Teikyo University Institute of Medical Mycology and ²Genome Research Center, Teikyo University, Hachioji, Tokyo, 192-0395 and ³Department of Pathobiology, School of Veterinary Medicine, Nihon University, Kanagawa 252-8510, Japan

(Received July 28, 2003. Accepted November 28, 2003)

*Corresponding author: Mailing address: Teikyo University Institute of Medical Mycology, 359 Otsuka, Hachioji, Tokyo 192-0395, Japan. Tel: +81-426-78-3256, Fax: +81-426-74-9190, E-mail: makimura@main.teikyo-u.ac.jp

SUMMARY: MEP3 of Microsporum canis encodes a 43.5 kDa extracellular keratinolytic metalloprotease, which is thought to be one of the virulence-related factors in dermatophytosis. In order to analyze the system underlying the regulation of MEP3 gene expression, the 5'-upstream region was isolated by inverse PCR. The nucleotide sequence of a DNA fragment of about 2.1 kb containing the coding region contains putative transcription factor binding sites and transcriptional initiation points. Further analyses of the regulatory sequence may be useful for understanding the molecular basis of the coordinated expression of the various genes involved in dermatophytosis.

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