Jpn. J. Infect. Dis., 57, 52-54, 2004

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Short Communication

Bactericidal Effects of Acidic Electrolyzed Water on the Dental Unit Waterline

Shinya Kohno*, Toshitsugu Kawata, Masato Kaku, Tadashi Fujita, Keisuke Tsutsui, Junji Ohtani, Kaoru Tenjo, Masahide Motokawa, Yuiko Tohma, Mao Shigekawa, Hiroko Kamata and Kazuo Tanne

Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical Sciences, Hiroshima 734-8553, Japan

(Received October 6, 2003. Accepted January 29, 2004)

*Corresponding author: Mailing address: Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical Sciences, Kasumi 1-2-3, Minami-ku, Hiroshima 734-8553, Japan. Tel: +81-82-257-5686, Fax: +81-82-257-5687, E-mail: acho@hiroshima-u.ac.jp

SUMMARY: Many studies have been conducted in the United States regarding the microbial contamination of dental unit waterline, but not in Japan. Recently, acidic electrolyzed water has been used in the medical and dental fields. In this study, we investigated the bactericidal effects of the temporary inflow of acidic electrolyzed water on microbial contamination of the dental unit waterline. First, in order to observe the daily bacterial contamination of the dental unit waterline, water samples were collected at the end of handpieces and three-way syringes before the inflow of acidic electrolyzed water. They were cultured to detect viable bacteria. Later, the inflow of acidic electrolyzed water was conducted through the piping box of the dental unit. Before starting operation on next day, water samples were collected and cultured, as described above. The mean viable bacteria count was 910 ± 190 CFU/ml at the end of handpieces, and 521 ± 116 CFU/ml at the end of three-way syringes before the inflow of acidic electrolyzed water. However, bacteria were detected in only small numbers at the end of handpieces and three-way syringes on the next day. These results indicated that acidic electrolyzed water could be applied as an appropriate measure against bacterial contamination of the dental unit waterline.

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