Jpn. J. Infect. Dis., 58, 115-118, 2005

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Short Communication

Detection of Rabies-Specific Antigens by Egg Yolk Antibody (IgY) to the Recombinant Rabies Virus Proteins Produced in Escherichia coli

Yurie Motoi, Satoshi Inoue¹*, Hajime Hatta², Kozue Sato, Kinjiro Morimoto³ and Akio Yamada¹

The United Graduate School of Veterinary Science, Gifu University, Gifu 501-1193, ¹Department of Veterinary Science and ³Department of Virology I, National Institute of Infectious Diseases, Tokyo 162-8640 and ²Department of Food and Nutrition, Faculty of Home Economics, Kyoto Women's University, Kyoto 605-0926, Japan

(Received November 30, 2004. Accepted January 19, 2005)

*Corresponding author: Mailing address: Laboratory of Transmission Control of Zoonosis, Department of Veterinary Science, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan. Tel: +81-3-5285-1111 ext. 2620, Fax: +81-3-5285-1179, E-mail: sinoue@nih.go.jp

SUMMARY: We obtained rabies-specific egg yolk antibodies (IgY) by immunizing hens with recombinant His-tagged nucleoprotein and phosphoprotein (rN, rP) of the rabies virus (CVS-11 strain) expressed in Escherichia coli. The anti-rN and rP IgY were shown to bind specifically to the respective proteins of the CVS-11 strain of rabies virus by Western blotting, immune fluorescent assay and immunohistochemistry, indicating that IgY to rabies recombinant proteins could serve as a reagent for diagnosis of rabies virus infection.

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