Jpn. J. Infect. Dis., 53 (2), 62-66, 2000

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Original Article

Effect of Sulfated Colominic Acid on Enteric Virus (Rotavirus, Poliovirus and Coxsackievirus) Infections In Vitro

Kyoko Konishi, Yanhong Gu¹, Ikumochi Hatano and Hiroshi Ushijima^1*

Department of Viral Diseases and Vaccine Control, National Institute of Infectious Diseases, Musashimurayama-shi, Tokyo 208-0011 and ¹Department of Developmental Medical Sciences, Institute of International Health, Graduate School of Medicine, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan

(Received January 28, 2000. Accepted May 11, 2000)

SUMMARY: Sulfated colominic acid exhibited suppressive effects on SA11 (simian rotavirus)- and MO (human rotavirus)-infections, but not on Wa (human rotavirus)-, Sabin 1 (poliovirus 1)-, and Nancy (coxsackie B3 virus)-infections, in vitro. The infection of SA11 was found to be inhibited by mixed treatment and early posttreatment with sulfated colominic acid, but not by pretreatment, by plaque assay and multiple growth assay. The results were confirmed by the infectivity titer, RNA polyacrylamide gel electrophoresis, and electron microscopic analysis. The mechanism of the suppressive effect was suggested to be adsorption inhibition at an early stage of the infection.

* Corresponding author: Tel/Fax: +81-3-5841-3629, E-mail: ushijima@m.u-tokyo.ac.jp

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