Jpn. J. Infect. Dis., 53 (2), 70-72, 2000
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Short Communication
Simultaneous Detection of Hepatitis B, C, and G Viral Genomes by Multiplex PCR Method
Nami Konomi1,3, Mari Yamaguchi2, Hideo
Naito1, Naoto Aiba1,
Takahide Saito3, Yasuyuki Arakawasup3 and
Kenji Abe1*
1Department of Pathology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku Tokyo 162-8640, 2Laboratory of Research and Development, Sanko Junyaku Co., Ltd., Inashiki Ibaraki 300-1155 and 33rd Department of Internal Medicine, Nihon University School of Medicine, Itabashi Tokyo 173-8610, Japan
(Received March 7, 2000. Accepted May 9, 2000)
SUMMARY: We established a multiplex polymerase chain reaction
(PCR) method for simultaneous detection of hepatitis B, C, and
G viral genomes. The levels of concordance with the data obtained
by conventional single PCR method were 100% for single infection,
98 to 100% for double infections, and 92% for triple infections.
This method is not only suited to rapid, large-scale epidemiological
screening and clinical diagnosis of those virus infections occurring
alone or in combination, but is also time- and cost-effective.
*Corresponding author: Tel: +81-3-5285-1111 ext. 2624, Fax: +81-3-5285-1189, E-mail: kenjiabe@nih.go.jp
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