Jpn. J. Infect. Dis., 53 (2), 70-72, 2000

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Short Communication

Simultaneous Detection of Hepatitis B, C, and G Viral Genomes by Multiplex PCR Method

Nami Konomi1,3, Mari Yamaguchi2, Hideo Naito1, Naoto Aiba1,
Takahide Saito3, Yasuyuki Arakawasup3 and Kenji Abe1*

1Department of Pathology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku Tokyo 162-8640, 2Laboratory of Research and Development, Sanko Junyaku Co., Ltd., Inashiki Ibaraki 300-1155 and 33rd Department of Internal Medicine, Nihon University School of Medicine, Itabashi Tokyo 173-8610, Japan

(Received March 7, 2000. Accepted May 9, 2000)


SUMMARY: We established a multiplex polymerase chain reaction (PCR) method for simultaneous detection of hepatitis B, C, and G viral genomes. The levels of concordance with the data obtained by conventional single PCR method were 100% for single infection, 98 to 100% for double infections, and 92% for triple infections. This method is not only suited to rapid, large-scale epidemiological screening and clinical diagnosis of those virus infections occurring alone or in combination, but is also time- and cost-effective.

*Corresponding author: Tel: +81-3-5285-1111 ext. 2624, 
Fax: +81-3-5285-1189, E-mail: kenjiabe@nih.go.jp

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