Jpn. J. Infect. Dis., 65 (4), 306-311, 2012

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Janisara Rudeeaneksin¹, Supranee Bunchoo¹, Sopa Srisungngam¹, Pathom Sawanpanyalert¹, Sawet Chamnangrom², Atipa Kamolwat², Porntip Thanasripakdeekul², Tooru Taniguchi³, Chie Nakajima⁴, Yasuhiko Suzuki^4,5*, and Benjawan Phetsuksiri¹

¹National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi; ²Regional Office of Disease Control 5, Department of Disease Control, Ministry of Public Health, Nakhon Ratchasima; ³Research Collaboration Center for Emerging and Re-emerging Diseases, Osaka University, Nonthaburi, Thailand; ⁴Division of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control, Sapporo 001-0020; and ⁵JICA/JST-SATREPS, Tokyo 120-8666, Japan

(Received April 5, 2012. Accepted May 16, 2012)

*Corresponding author: Mailing address: Department of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control, Kita 20, Nishi 10, Kita-ku, Sapporo 001-0020, Japan. Tel: +81-11-706-9503, Fax: +81-706-7310, E-mail: このメールアドレスはスパムボットから保護されています。閲覧するにはJavaScriptを有効にする必要があります。

SUMMARY: Definitive diagnosis of tuberculosis (TB) by conventional culture, followed by bacterial identification based on biochemical tests is time-consuming and tedious. Simple loop-mediated isothermal amplification (LAMP) specific for Mycobacterium tuberculosis complex, targeting the M. tuberculosis 16S ribosomal RNA gene, termed TB-LAMP, was evaluated as an alternative for rapid culture confirmation. TB-LAMP was assessed for its ability to detect M. tuberculosis complex in BACTEC MGIT 960-positive cultures. Of the 103 cultures evaluated, 100 were identified to contain M. tuberculosis complex by TB-LAMP and had concordant results with standard biochemical tests of niacin accumulation, nitrate reductase, lack of heat-stable catalase, and susceptibility to para-nitrobenzoic acid. These results indicate that TB-LAMP in combination with BACTEC MGIT 960 is a specific, reliable, and technically feasible method for rapid and accurate identification of M. tuberculosis complex.