1. Shimizu Y, Shinoda T, Shirasago Y, Kondoh M, Shinya N, Hanada K, Yagi K, Suzuki T, Wakita T, Kimura-Someya T, Shirouzu M, and *Fukasawa M (2021) Generation and characterization of single-chain variable fragment occludin-binding antibodies to prevent hepatitis C virus infection, FEBS Let, 595, 220-229. doi: 10.1002/1873-3468.13975.
  2. Ogawa M, Shirasago Y, Tanida I, Kakuta S, Uchiyama Y, Shimojima M, Hanada K, Saijo M, and *Fukasawa M (2021)Structural basis of antiviral activity of caffeic acid against severe fever with thrombocytopenia syndrome virus, J Infect Chemother, 27, 397-400. doi: 10.1016/j.jiac.2020.10.015.
  3.  *Rizzo R, Russo D, Kurokawa K, Sahu P, Lombardi B, Supino D, Zhukovsky M, Vocat A, Pothukuchi P, Kunnathully V, Capolupo L, Boncompain G, Vitagliano C, Marino FZ, Aquino G, Montariello D, Henklein P, Mandrich L, Botii G, Clausen H, Mandel U, Yamaji T, Hanada K, Budillon A, Perez F, Parashuraman S, Hannun YA, Nakano A, Corda D, *D’Angelo G and *Luini A (2021) Golgi maturation-dependent glycoenzyme recycling controls glycosphingolipid biosynthesis and cell growth via GOLPH3. EMBO Journal. 2021, 40, e107238, Open access: https://www.embopress.org/doi/epdf/10.15252/embj.2020107238
  4. Okemoto-Nakamura Y, Someya K, Yamaji T, Saito K, Takeda M, and *Hanada K (2021) Poliovirus-nonsusceptible Vero cell line for the World Health Organization global action plan, Sci Rep, 11, article 6746. Open access: https://www.nature.com/articles/s41598-021-86050-3 (Y.O.-N.. and K.S. are co-first authors)
  5. Sakuma C, Sekizuka T, Kuroda M, Hanada K, and *Yamaji T (2021) Identification of SYS1 as a host factor required for Shiga toxin-mediated cytotoxicity in Vero cells, Int J Mol Sci, 22, article 4936. Open access: https://doi.org/10.3390/ijms22094936
  6. Yamanaka A, Matsuda M, Okabayashi T, Pitaksajjakul P, Ramasoota P, Saito K, Fukasawa M, Hanada K, Matsuura T, Muramatsu M, Shioda T, and *Suzuki R (2021) Seroprevalence of flavivirus neutralizing antibodies in Thailand by high-throughput neutralization assay: Endemic circulation of Zika virus before 2012, mSphere, 6, e00339-21. Open access: https://doi.org/10.1128/mSphere.00339-21 (A.Y. & M.M. are co-first authors)
  7. *Furuichi K, Hiraoka M, Hanada K, Fujiwara T, and *Kojima C (2021) Sequence requirements of the FFAT-like motif for specific binding to VAP-A are revealed by NMR, FEBS Lett, 595, 2248-2256. doi: 10.1002/1873-3468.14166.
  8. Tamura N, Sakai S, Martorell L, Colomé R, Mizuike A, Goto A, *Ortigoza-Escobar JD, and *Hanada K (2021) Intellectual disability-associated mutations in the ceramide transport protein gene CERT1 lead to aberrant function and subcellular distribution, J Biol Chem, 297, article 101338. Open access: https://doi.org/10.1016/j.jbc.2021.101338


  1. Tachida Y, Kumagai K, Sakai S, Ando S, *Yamaji T, and *Hanada K (2020) Chlamydia trachomatis-infected human cells convert ceramide to sphingomyelin without sphingomyelin synthases 1 and 2. FEBS Let, 594, 519-529. doi.org/10.1002/1873-3468.13632
  2.   *Hanada K (2020) Organelle contacts: sub-organelle zones to facilitate rapid and accurate inter-organelle trafficking of lipids.Traffic, 21, 189-196. doi.org/10.1111/tra.12716  (invited commentary)
  3. Ueno M, Miyoshi N, Hanada K, and *Kobayashi S (2020) Three-component, one-pot tandem Sonogashira/Suzuki–Miyaura coupling reactions for the synthesis of a library of ceramide-transport protein inhibitors designed in silico, Asia J Org Chem, 9, 267-273. DOI: 10.1002/ajoc.201900689
  4. *Saito K, Fukasawa M, Shirasago Y, Suzuki R, Osada N, Yamaji T, Wakita T, Konishi E, and Hanada K (2020) Comparative characterization of flavivirus production in two cell lines: Human hepatoma-derived Huh7.5.1-8 and African green monkey kidney-derived Vero. PLoS ONE, 15, e0232274. https://doi.org/10.1371/journal.pone.0232274
  5.  Morimoto K, Suzuki N, Tanida I, Kakuta S, Furuta Y, Uchiyama Y, Hanada K, Suzuki Y, *Yamaj T (2020) Blood group P1 antigen-bearing glycoproteins are functional but less efficient receptors of Shiga toxin than conventional glycolipid-based receptors, J Biol Chem, 295, 9490-9501. doi: 10.1074/jbc.RA120.013926.
  6.  Goto A, Mizuike A, and *Hanada K (2020) Sphingolipid metabolism occurring at the ER-Golgi contact zone and its impact on membrane trafficking, CONTACT, 3, article 2515256420959514. Open access: 10.1177/2515256420959514 (invited review article) (A.G. and A.M. are co-first authors)
  7. Gewaid H, Aoyagi H, Arita M, Watashi K, Suzuki R, Sakai S, Kumagai K, Yamaji T, Fukasawa M, Kato F, Hishiki T, Mimata A, Sakamaki Y, Ichinose S, Hanada K, Muramatsu M, Wakita T, and *Aizaki (2020) Sphingomyelin is essential for the structure and function of the double-membrane vesicles in the viral RNA replication factories, double-membrane vesicles, J Virol, 94, e01080-20. https://doi.org/10.1128/JVI.01080-20.
  8. Kawamoto M, Yamaji T, Saito K, Shirasago Y, Satomura K, Endo T, Fukasawa M, *Hanada K, and *Osada N (2020) Identification of characteristic genomic markers in human hepatoma HuH-7 and Huh7.5.1-8 cell lines, Frontier in Genetics, 111, Article 546106. Open access: https://doi.org/10.3389/fgene.2020.546106
  9. Murakami H, Tamura N, Enomoto Y, Shimasaki K, *Kurosawa K, and *Hanada K (2020) Intellectual disability-associated gain-of-function mutations in CERT1 that encodes the ceramide transport protein CERT, PLoS ONE, 15, e0243980. (H.M. and N.T. are co-first authors. K.K. and K.H. are co-correspondence) Open access: https://doi.org/10.1371/journal.pone.0243980


  1. *Yamaji T, Sekizuka T, Tachida Y, Sakuma C, Kuroda M, and Hanada K (2019) A CRISPR screen identifies LAPTM4A and TM9SF proteins as glycolipid-regulating factors, iScience, 11, 409-424. doi.org/10.1016/j.isci.2018.12.039.
  2. Nakao N, Ueno M, Sakai S, Egawa D, Hanzawa H, Kawasaki S, Kumagai K, Suzuki M, *Kobayashi S, and *Hanada K (2019) Natural ligand-nonmimetic inhibitors to the lipid transfer protein CERT, Commun Chem, 2, article 20.doi: 10.1038/s42004-019-0118-3 (N.N., M.U., and S.S. are co-first authors) (open access: https://www.nature.com/articles/s42004-019-0118-3)
  3. Shirasago Y, Inamori Y, Suzuki T, Tanida I, Suzuki T, Sugiyama K, Wakita T, Hanada K, and *Fukasawa M (2019) Inhibition mechanisms of hepatitis C virus infection by caffeic acid and tannic acid, Biol Pharm Bull, 42, 770. doi:10.1248/bpb.b18-00970 (a Highlighted paper selected by Editor-in-Chief).
  4. Shimizu Y, Shirasago Y, Suzuki T, Hata T, Kondoh M, Hanada K, Yagi K, and *Fukasawa M (2019) Characterization of monoclonal antibodies recognizing each extracellular loop domain of occluding, J Biochem, 166, 297-308. doi: 10.1093/jb/mvz037
  5. *Yamaji T, Hanamatsu H, Sekizuka T, Kuroda M, Ohnishi M,Furukawa J, *Yahiro K,, and Hanada K (2019) A CRISPR screen using subtilase cytotoxin identifies SLC39A9 as a glycan-regulating factor, iScience, 15, 407-420. doi: 10.1016/j.isci.2019.05.005
  6.   *Kumagai K, and *Hanada K (2019) Structure, functions, and regulation of CERT, a lipid-transfer protein for the delivery of ceramide at the ER-Golgi membrane contact sites, FEBS Let, 593, 2366-2377. https://doi.org/10.1002/1873-3468.13511 (invited review) 
  7. *Osawa T, *Shimamura T, Saito K, Hasegawa Y, Ishii N, Nishida M, Kondo A, Anwar M, Tsuchida R,Hino S, Sakamoto A, Igarashi K, Saitoh K, Kato K, Endo K, Yamano S, Kanki Y, Matsumura Y, Minami T, Tanaka T, Anai M, Wada Y, Wanibuchi H, Hayashi M, Hamada A, Yoshida M, Yachida S, Nakao M, Sakai J, Aburatani H, Shibuya M, Hanada K, Miyano S, *Soga T, and *Kodama T (2019) Phosphoethanolamine accumulation protects cancer cells under glutamine starvation through downregulation of PCYT2. Cell Rep, 29, 89-103. doi.org/10.1016/j.celrep.2019.08.087
  8. *Okemoto-Nakamura Y, Tanida I, Yamaji T, *Hanada K, and Hagiwara K (2019) A PRNP-disrupted human neuroblastoma cell line and its stable transformants expressing human prion variants. BPB Rep, 2, 73-79. (Open access: https://www3.e-kenkyu.com/bpb-reports-online-journal/uploads/manuscript/file/38/2_73.pdf).


  1. Sakuma C, Sekizuka T, Kuroda M, Kasai F, Saito K, Ikeda M, Yamaji T, *Osada N, and *Hanada K (2018) Novel endogenous simian retroviral integrations in Vero cells: implications for quality control of a human vaccine cell substrate, Sci Rep, 8, 644. (C.S. and T.S. are co-first authors. N.O. and K.H. are co-correspondence) doi: 10.1038/s41598-017-18934-2. (Open access: https://www.nature.com/articles/s41598-017-18934-2)
  2. Shimasaki K, Watanabe-Takahash M, Umeda M, Funamoto S, Saito Y, Noguchi N, Kumagai K, Hanada K, Tsukahara F, Maru Y, Shibata N, Naito M, and *Nishikawa K (2018) Pleckstrin homology domain of p210 BCR-ABL interacts with cardiolipin to regulate its mitochondrial translocation and subsequent mitophagy, Genes Cells, 23, 22-34. doi: 10.1111/gtc.12544.
  3. Otsuki N, Sakata M, Saito K, Okamoto K, Mori Y, *Hanada K, and *Takeda M (2018)  Both sphingomyelin and cholesterol in the host cell membrane are essential for Rubella virus entry. J Virol, 92, e01130-17. doi: 10.1128/JVI.01130-17.(selected as Spotlight)
  4. Shimizu Y, Shirasago Y, Kondoh M, Suzuki T, Wakita T, Hanada K, Yagi K, and *Fukasawa M (2018) Monoclonal antibodies against occludin completely prevented hepatitis C virus infection in a mouse model, J Virol, 92, e02258-17. doi:10.1128/JVI.02258-17. (selected as Spotlight)
  5. Sugiki T, Egawa D, Kumagai K, Kojima C, Fujiwara T, Takeuchi K, Shimada I, *Hanada K, and *Takahashi H (2018) Phosphoinositide binding by the PH domain in ceramide transfer protein (CERT) is inhibited by hyperphosphorylation of an adjacent serine-repeat motif, J Biol Chem, 293, 11206-11217. (T.S. and D.E. are co-first authors. K.H. and H.T. are co-correspondence) doi: 10.1074/jbc.RA118.002465.
  6. Ikeda M, Satomura K, Sekizuka T, Hanada K, Endo T, *Osada N (2018) Comprehensive phylogenomic analysis reveals a novel cluster of simian endogenous retroviral sequences in Colobinae monkeys, Am J Primatol, e22882. doi: 10.1002/ajp.22882.
  7. *Hanada K (2018) Lipid-transfer proteins rectify inter-organelle flux and accurately deliver lipids at membrane contact sites, J Lipid Res, 59, 1341-1366. doi:  org/10.1194/jlr.R085324 (Open access: http://www.jlr.org/content/59/8/1341.full.pdf+html(invited review)
  8. *Saito K, Otsuki N, Takeda M, and Hanada K (2018) Liposome flotation assay for studying interactions between Rubella virus particles and lipid membranes, Bio-Protocol, 8(16), e2983.
  9. Shirasago Y, Fukazawa H, Aizaki H, Suzuki T, Suzuki T, Sugiyama K, Wakita T, Hanada K, Abe R, *Fukasawa M (2018) Thermostable hepatitis C virus JFH1-derived variant isolated by adaptation to Huh7.5.1 cells, J Gen Virol, 99(10), 1407-1417. doi: 10.1099/jgv.0.001117.
  10. *Kumagai K, Elwell CA, Andoh S, Engel JN, *Hanada K (2018) Both the N- and C- terminal regions of the Chlamydial inclusion protein D (IncD) are required for interaction with the pleckstrin homology domain of the ceramide transport protein CERT, Biochem Biophys Res Commun, 505(4), 1070-1076. doi: 10.1016/j.bbrc.2018.09.168.

Copyright 1998 National Institute of Infectious Diseases, Japan

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